Journal: Food Science & Nutrition

Article Title: Panax notoginseng Saponins Accelerate Diabetic Wound Repair by Minimizing Mitochondrial Dysfunction and Inhibiting Pyroptosis of Macrophage via Regulating Ephx2/11,12‐ EET Axis

doi: 10.1002/fsn3.72494

Figure Lengend Snippet: PNS inhibits pyroptosis and mitochondrial homeostasis in wound tissue of DW mice. (A–D) ELISA was used to measure serum levels of IL‐1β, IL‐6, IL‐18, and IL‐10. (E) Immunofluorescence staining of wound sections identified M1 macrophages using antibodies against F4/80 and iNOS. Scale bar: 200 μm. (F) Immunofluorescence staining of wound sections identified M2 macrophages using antibodies against F4/80 and Arg1. Scale bar: 200 μm. (G, H) Western blotting detected protein expression of iNOS and Arg1 in wound tissues, followed by quantitative analysis. (I–K) Western blotting detected protein expression of NLRP3, Casp1, Casp1 p20, FL‐GSDMD, and N‐GSDMD in wound tissues, followed by quantitative analysis. (L, M) Immunohistochemical staining and quantitative analysis of GSDMD expression in wound sections. (N, O) Western blotting detected protein expression of Mfn1, Mfn2, OPA1, p‐Drp1, and Drp1 in wound tissues, followed by quantitative analysis. Values were expressed as means ± SEM, n = 6–8. ** p < 0.01, vs. NW; # p < 0.05, ## p < 0.01 vs. DW.

Article Snippet: For double immunofluorescence staining, sections were incubated overnight at 4°C with primary antibodies against F4/80 (a macrophage marker; 1:100; Santa Cruz, sc‐377009) in combination with either iNOS (an M1 marker; 1:100; ABclonal, A0312) or Arg1 (an M2 marker; 1:100; ABclonal, A1847).

Techniques: Tissue, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Staining, Western Blot, Expressing, Analysis, Immunohistochemical staining